利用Dualex植物葉綠素儀研究配位體比率對大豆植株錳吸收的影響

更新時間：2022-05-09　點擊量：2430

Dualex是一款源自于法國國家科學院 (CNRS)及巴黎第十一大學技術，由奧地利PESSL公司生產（原法國Force-A公司）開發的新型多功能葉片測量儀。它可同時準確測量葉片的葉綠素含量、葉片表層的類黃酮和花青素含量，適用于植物生理學和農學（如水稻葉綠素濃度，玉米氮素狀況，葡萄藤等）相關研究。

其測量對象可以是單子葉植物，雙子葉植物或多年生植物。這款設備簡單易用，可進行實時和非破壞性測量。由于不需要校準標定和事先的樣品制備，測量工作可在實驗室或現場完成。此外，該設備在各種溫度的和環境光照條件下均可正常使用。系統光學傳感器可進行簡單、快速、無損測量葉片中的葉綠素、多酚以及花青素。

Dualex便攜式智能氣象-葉綠素-花青素-黃酮醇測量儀

摘要

背景：應用合成螯合物的環境風險進一步推動了可生物降解復合物的實施，以補充植物錳元缺乏。本研究使用可生物降解的七葡萄糖酸鹽（G7）和葡萄糖酸鹽（G6）配體來測試Mn2+：配體比例對其肥料向植物提供錳的能力的影響。在水培和石灰性土壤條件下，評估了這些復合物糾正缺錳大豆的效果，并與合成螯合物EDTA（乙二胺四乙酸）進行了比較。

結果：本研究表明，G7是EDTA的可生物降解替代品，用于供應錳，與G6相比，G7保持了充足的營養平衡，從而減少了植物對鐵（Fe）的吸收。Mn配合物的功效取決于配體和Mn:配體比率，根據其化學結構和穩定性，Mn2+：G7的1:1和1:2摩爾比在短期內是*的配合物。

結論：Mn2+：G7（1:1和1:2）配合物的化學結構在堿性溶液中具有足夠的穩定性和快速作用，是植物營養的有效錳源。

關鍵詞：甘氨酸；缺錳植物；Mn：配體比例；生物可降解配體；葡萄糖酸鹽；七葡萄糖酸鹽。

Implications of the Mn:ligand ratio for Mn uptake by Glycine max L. plants fertilized with heptagluconate and gluconate complexes

Abstract

Background: The environmental risk of the application of synthetic chelates has furthered the implementation of biodegradable complexes to correct manganese (Mn)-deficient plants. This study used the biodegradable ligands of heptagluconate (G7) and gluconate (G6) to test the influence of the Mn2+ :ligand ratio on their fertilizers' capacity to provide Mn to plants. The efficacy of these complexes to correct Mn-deficient soybean was evaluated in hydroponics and calcareous soil conditions and compared with the synthetic chelate EDTA (ethylenediaminetetraacetic acid).

Results: This study demonstrated that G7 was a biodegradable alternative to EDTA for supplying Mn, maintaining an adequate nutritional balance compared with G6, which reduced iron (Fe) uptake by the plants. The efficacy of the Mn complexes depended on both the ligand and the Mn:ligand ratio, with the 1:1 and 1:2 molar ratios of Mn2+ :G7 being the most effective complexes in the short term on the basis of their chemical structure and stability.

Conclusion: The Mn2+ :G7 (1:1 and 1:2) complexes were found to be effective Mn sources for plant nutrition due to their chemical structures providing adequate stability in alkaline solution and their fast-action effect. © 2021 Society of Chemical Industry.

Keywords: Glycine max L.; Mn-deficient plants; Mn:ligand ratio; biodegradable ligands; gluconate; heptagluconate.